5GHE

Crystal Structure of Bacillus thuringiensis Cry6Aa2 Protoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9200
Spacegroup name	P 65
Unit cell lengths	101.637, 101.637, 78.905
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.010 - 1.901
R-factor	0.1687
Rwork	0.167
R-free	0.19350
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.762
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.900
Number of reflections	36545
<I/σ(I)>	17.2
Completeness [%]	99.8
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Glycerol, PEG 100, Tris-HCl