5GGY

X-ray crystal structure of Periplasmic Desferal binding protein FhuD from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-09
Detector	RAYONIX MX-225
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	57.246, 71.746, 125.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.316 - 2.500
R-factor	0.1926
Rwork	0.190
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efd
RMSD bond length	0.003
RMSD bond angle	0.729
Data scaling software	SCALA
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.320	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	18616
<I/σ(I)>	9.1
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18%(w/v) PEG 6000, 0.1 M MES (pH 6.0), 0.1M Tris (pH 7)