5GGW
Crystal structure of Class C beta-lactamase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.732, 182.088, 141.881 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.436 - 1.762 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.25890 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.148 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.760 |
Number of reflections | 100735 |
<I/σ(I)> | 21.9 |
Completeness [%] | 94.7 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 295 | Bis-Tris pH 5.5 Ammonium sulfate |