5GGL
Crystal structure of N-terminal domain of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with GlcNAc-alpha-pNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 53.330, 68.686, 88.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.124 - 1.270 |
R-factor | 0.1451 |
Rwork | 0.145 |
R-free | 0.16060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ggg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.284 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.100 | 1.290 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.090 | 1.840 |
Number of reflections | 86073 | |
<I/σ(I)> | 14.4 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.7 | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | Tris-HCl, PEG-8000 |