5GGK
Crystal structure of N-terminal domain of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with Man-beta-pNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 53.358, 70.129, 89.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.065 - 1.300 |
| R-factor | 0.1469 |
| Rwork | 0.146 |
| R-free | 0.17550 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 83588 | |
| <I/σ(I)> | 18.9 | 1.4 |
| Completeness [%] | 99.9 | 97.5 |
| Redundancy | 12.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Tris-HCl, PEG-10000 |
