5GGK

Crystal structure of N-terminal domain of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase in complex with Man-beta-pNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1000
Spacegroup name	P 2 21 21
Unit cell lengths	53.358, 70.129, 89.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.065 - 1.300
R-factor	0.1469
Rwork	0.146
R-free	0.17550
RMSD bond length	0.008
RMSD bond angle	1.047
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	83588
<I/σ(I)>	18.9	1.4
Completeness [%]	99.9	97.5
Redundancy	12.4	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Tris-HCl, PEG-10000