5GGF
Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-21 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 168.688, 186.444, 143.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.611 - 2.490 |
R-factor | 0.2335 |
Rwork | 0.233 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.158 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.610 | 2.550 |
High resolution limit [Å] | 2.497 | 2.500 |
Rmerge | 0.112 | 1.200 |
Number of reflections | 79199 | |
<I/σ(I)> | 16.13 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.4-1.6M Na malonate |