5GGE

Fatty Acid-Binding Protein in Brain Tissue of Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	110
Detector technology	CCD
Collection date	2013-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	61.698, 61.698, 137.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.052 - 1.861
R-factor	0.1855
Rwork	0.179
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftp
RMSD bond length	0.012
RMSD bond angle	1.297
Data reduction software	SCALEPACK
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.860	1.860
Rmerge	0.068	0.503
Number of reflections	11582
<I/σ(I)>	11.5	2.96
Completeness [%]	99.5	99.3
Redundancy	6.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	2M ammonia sulfate, 100mM phosphate citrate, pH4.2