5GAI
Probabilistic Structural Models of Mature P22 Bacteriophage Portal, Hub, and Tailspike proteins
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.1 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN ULTRASCAN 10000 (10k x 10k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 10.5 |
| Number of Particle | 79731 |
| Software | ETHAN,MPSA,EMAN |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
