5G5Y
S.pneumoniae ABC-transporter substrate binding protein FusA apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.070, 72.170, 77.660 |
| Unit cell angles | 108.02, 104.99, 92.49 |
Refinement procedure
| Resolution | 70.760 - 1.730 |
| R-factor | 0.16245 |
| Rwork | 0.161 |
| R-free | 0.19317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.473 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.010 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.060 | 0.920 |
| Number of reflections | 114100 | |
| <I/σ(I)> | 11.6 | 1.6 |
| Completeness [%] | 83.7 | 33.8 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 15 % PEG6000, 0.1M TRIS-HCL PH 8.0, 0.01 M ZINC CHLORIDE |
