5G5R
CBS domain tandem of site-2 protease from Archaeoglobus fulgidus in complex with llama Nanobody - apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2013-11-30 |
Detector | DECTRIS PLIATUS 2M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 68.440, 68.440, 196.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.979 - 2.400 |
R-factor | 0.2093 |
Rwork | 0.207 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4FHB AND 3KPB |
RMSD bond length | 0.002 |
RMSD bond angle | 0.443 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.180 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.880 |
Number of reflections | 11381 | |
<I/σ(I)> | 24.24 | 2.31 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 9.16 | 8.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | WITH 0.14 M BIS-TRIS PH 6.0, 0.28 M (NH4)2SO4 AND 19% PEG 3350 |