5G5O
Structure of the snake adenovirus 1 hexon-interlacing LH3 protein, native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.790, 126.880, 120.940 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.17305 |
| Rwork | 0.172 |
| R-free | 0.22569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g5n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.200 | 0.720 |
| Number of reflections | 130350 | |
| <I/σ(I)> | 3.8 | 1.4 |
| Completeness [%] | 94.3 | 87.6 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 MM TRIS-HCL PH 8.5, 20% (W/V) PEG3350, 0.2 M AMMONIUM ACETATE |
