5G5N
Structure of the snake adenovirus 1 hexon-interlacing LH3 protein, methylmercury chloride derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 |
| Unit cell lengths | 149.259, 149.259, 108.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 2.300 |
| R-factor | 0.17317 |
| Rwork | 0.172 |
| R-free | 0.20466 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.521 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 52927 | |
| <I/σ(I)> | 14.2 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 MM TRIS-HCL, 5% (V/V) GLYCEROL, 24% (W/V) PEG4000, 50 MM MOPS-NAOH PH 7.0. |
