5G5H
Escherichia coli Periplasmic Aldehyde Oxidase R440H mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-29 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.839, 78.263, 151.732 |
| Unit cell angles | 90.00, 99.93, 90.00 |
Refinement procedure
| Resolution | 48.270 - 2.300 |
| R-factor | 0.16664 |
| Rwork | 0.164 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1RM6 1t3q 1ffu 1ffv |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.857 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.210 | 0.830 |
| Number of reflections | 55360 | |
| <I/σ(I)> | 8.7 | 1.81 |
| Completeness [%] | 98.0 | 84.2 |
| Redundancy | 7.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG 3350, AMMONIUM IODIDE, pH 7.5 |
