5G56

THE TETRA-MODULAR CELLULOSOMAL ARABINOXYLANASE CtXyl5A STRUCTURE AS REVEALED BY X-RAY CRYSTALLOGRAPHY

Replaces:  5AK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-04
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	147.410, 191.730, 50.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.700 - 2.640
R-factor	0.23928
Rwork	0.237
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2Y8K 3mpc 2Y8M 2Y9I AND 2Y9S
RMSD bond length	0.008
RMSD bond angle	1.211
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.700	2.780
High resolution limit [Å]	2.640	2.640
Rmerge	0.160	0.700
Number of reflections	42246
<I/σ(I)>	8	2
Completeness [%]	98.5	96.4
Redundancy	5.8	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		40% (V/V) 2-METHYL-2-4PENTANEDIOL (MPD), pH 7