5G52
Crystallographic structure of full particle of Deformed Wing Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 360.131, 360.131, 360.131 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.805 - 3.802 |
| R-factor | 0.2849 |
| Rwork | 0.283 |
| R-free | 0.32240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.466 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 3.910 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.370 | 0.800 |
| Number of reflections | 56889 | |
| <I/σ(I)> | 2 | 0.9 |
| Completeness [%] | 75.7 | 76.1 |
| Redundancy | 1.8 | 1.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLIZED FROM 0.8 M POTASSIUM DIHYDROGEN PHOSPHATE, 0.8 M SODIUM DIHYDROGEN PHOSPHATE, 0.1 M SODIUM HEPES, 7.5 PH |
