5G51
High resolution structure of the part of VP3 protein of Deformed Wing Virus forming P-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 63.976, 104.922, 57.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.749 - 1.450 |
| R-factor | 0.1899 |
| Rwork | 0.189 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.047 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.750 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 34920 | |
| <I/σ(I)> | 14.2 | 2.1 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 6.09 | 5.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 4.3 M NACL, |
