5G50
Vibrio cholerae scaffolding protein RbmA in complex with magnesium.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A |
Synchrotron site | NSRRC |
Beamline | BL15A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-27 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 123.513, 123.513, 100.526 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.560 - 2.300 |
R-factor | 0.20328 |
Rwork | 0.202 |
R-free | 0.23714 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4be6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.482 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.560 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.030 | 0.410 |
Number of reflections | 34970 | |
<I/σ(I)> | 17.16 | 1.9 |
Completeness [%] | 100.0 | 98 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1 M BIS-TRIS, PH 6, 0.2 M NACL, 10 MM MGSO4 |