5G50
Vibrio cholerae scaffolding protein RbmA in complex with magnesium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A |
| Synchrotron site | NSRRC |
| Beamline | BL15A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 123.513, 123.513, 100.526 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.560 - 2.300 |
| R-factor | 0.20328 |
| Rwork | 0.202 |
| R-free | 0.23714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4be6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.482 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | 0.410 |
| Number of reflections | 34970 | |
| <I/σ(I)> | 17.16 | 1.9 |
| Completeness [%] | 100.0 | 98 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M BIS-TRIS, PH 6, 0.2 M NACL, 10 MM MGSO4 |
