5G4Y
Structural basis for carboxylic acid recognition by a Cache chemosensory domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-11 |
Detector | ADSC CCD |
Spacegroup name | P 64 |
Unit cell lengths | 74.282, 74.282, 75.057 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.330 - 2.000 |
R-factor | 0.16196 |
Rwork | 0.161 |
R-free | 0.18315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k08 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.857 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.760 |
Number of reflections | 16085 | |
<I/σ(I)> | 14.2 | 2.9 |
Completeness [%] | 99.8 | 97.8 |
Redundancy | 11.2 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PROTEIN AT 8.5 MG/ML IN 50 MM BIS-TRIS CHLORIDE, PH 6.5, 50 MM SODIUM CHLORIDE IN EQUAL VOLUME WITH 27% PEG 4000 AND 100 MM PROLINE AT 20 C IN SITTING DROPS. |