5G4T
The structure of a quasi-cyclic six k-turn duplex RNA species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-02 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 70.306, 70.306, 47.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.611 - 2.751 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cs1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.330 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.040 | 1.500 |
| Number of reflections | 2019 | |
| <I/σ(I)> | 27.7 | 0.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.6 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 3.5 M NA FORMATE, 0.1 M NA ACETATE (PH 4.6) |
