5G4L
Phloroglucinol reductase from Clostridium sp. with bound NADPH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-14 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.686, 123.083, 55.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.030 - 1.800 |
R-factor | 0.1559 |
Rwork | 0.155 |
R-free | 0.17920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g4k |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.140 | 1.170 |
Number of reflections | 57138 | |
<I/σ(I)> | 17 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.2 | 13.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 28 % PEG 3350 0.1 M BIS-TRIS, PH 7.2 LI2SO4 3 MM NADPH |