5G4K
Phloroglucinol reductase from Clostridium sp. apo-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-19 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.756, 125.391, 55.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.980 - 1.740 |
R-factor | 0.1582 |
Rwork | 0.157 |
R-free | 0.18180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hq1 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.980 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.110 | 0.870 |
Number of reflections | 65090 | |
<I/σ(I)> | 13.7 | 2.4 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 8.2 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.75 | 31% PEG 3350 0.1 M BIS-TRIS, PH 5.75 LI2SO4 |