5G4J
Phospholyase A1RDF1 from Arthrobacter in complex with phosphoethanolamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.166, 96.371, 121.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.600 - 1.870 |
| R-factor | 0.16884 |
| Rwork | 0.167 |
| R-free | 0.19943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g4i |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.915 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.590 | 1.950 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 74689 | |
| <I/σ(I)> | 14.5 | 2.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 24% (W/V) PEG 3350, 0.2 M (NH4)2SO4 AND 3% (V/V) 2-METHYL-2,4-PENTANEDIOL IN 0.1 M BIS-TRIS PROPANE BUFFER AT PH 6.0 |
