5G4I
PLP-dependent phospholyase A1RDF1 from Arthrobacter aurescens TC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2015-11-28 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.937, 97.176, 124.261 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.550 - 1.500 |
R-factor | 0.1543 |
Rwork | 0.153 |
R-free | 0.17549 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.027 |
RMSD bond angle | 2.251 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.550 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.530 |
Number of reflections | 148178 | |
<I/σ(I)> | 21.6 | 4.1 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 8.2 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 24% (W/V) PEG 3350, 0.2 M (NH4)2SO4 AND 3% (V/V) 2-METHYL-2,4-PENTANEDIOL IN 0.1 M BIS-TRIS PROPANE BUFFER AT PH 6.0 |