5G4C
Human SIRT2 catalyse short chain fatty acyl lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.341, 117.310, 71.346 |
| Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| Rwork | 0.183 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zgo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.000 | 2.100 |
| Rmerge | 0.100 | 0.218 |
| Number of reflections | 40356 | |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 12% PEG 8K, 0.1 M HEPES, PH 7.5, 5% ISOPROPANOL |
