5G48
H.pylori Beta clamp in complex with Diflunisal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2015-11-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.311, 66.372, 89.119 |
| Unit cell angles | 90.00, 115.96, 90.00 |
Refinement procedure
| Resolution | 33.190 - 2.280 |
| R-factor | 0.23558 |
| Rwork | 0.235 |
| R-free | 0.25475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rki |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.169 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.130 | 80.130 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 39385 | |
| <I/σ(I)> | 15.1 | 1.9 |
| Completeness [%] | 99.1 | 93.6 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6% W/V PEG 20,000, 20% V/V PEG 550MME, 0.01M MGCL2, 0.1M MOPS/ HEPES-NA PH7.3, 0.2M AMMONIUM CITRATE |
