5G3X
Structure of recombinant granulovirus polyhedrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-05 |
Detector | MARRESEARCH |
Spacegroup name | I 2 3 |
Unit cell lengths | 102.058, 102.058, 102.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.276 - 1.660 |
R-factor | 0.1535 |
Rwork | 0.149 |
R-free | 0.19220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jvb |
RMSD bond length | 0.007 |
RMSD bond angle | 0.862 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.280 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Rmerge | 0.260 | |
Number of reflections | 21009 | |
<I/σ(I)> | 7.5 | 1.5 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8.7 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | IN VIVO CRYSTALLIZATION, pH 7.0 |