5G3Q
Crystal structure of a hypothetical domain in WNK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.238, 70.324, 82.255 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.502 - 1.613 |
| R-factor | 0.2028 |
| Rwork | 0.202 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNRELEASED SAD-DETERMINED SELENOMETHIOINE MODEL |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.621 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.300 | 1.620 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.030 | 0.690 |
| Number of reflections | 30010 | |
| <I/σ(I)> | 27 | 2.3 |
| Completeness [%] | 99.9 | 94.8 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350 -- 10% ETHYLENE GLYCOL -- 0.1M BIS-TRIS-PROPANE PH 6.5 -- 0.2M SODIUM FORMATE |
