5G3G
Preserving MEtallic Sites Affected by Radiation Damage the CuT2 case in Thermus Thermophilus multicopper Oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 287 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.744, 110.332, 96.484 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.707 - 1.840 |
R-factor | 0.1462 |
Rwork | 0.145 |
R-free | 0.17570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yae |
RMSD bond length | 0.007 |
RMSD bond angle | 0.983 |
Phasing software | REFMAC |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.720 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.590 |
Number of reflections | 45713 | |
Completeness [%] | 98.3 | 96.7 |
Redundancy | 6.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |