5G3C
Preserving Metallic sites affected by radiation damage the CuT2 case in thermus termophilus multicopper oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.744, 110.332, 96.484 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.660 - 1.750 |
| R-factor | 0.1371 |
| Rwork | 0.136 |
| R-free | 0.16570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yae |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.090 | |
| Number of reflections | 393350 | |
| Completeness [%] | 98.1 | 96.1 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
