5G36

Yellow form of Halorhodopsin from Halobacterium salinarum in a new rhombohedral crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-05
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3 2
Unit cell lengths	103.000, 103.000, 136.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.393 - 2.600
R-factor	0.1943
Rwork	0.191
R-free	0.25270
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	2.730
High resolution limit [Å]	2.600	2.600
Rmerge	0.160	0.910
Number of reflections	8774
<I/σ(I)>	14.5	1.8
Completeness [%]	99.9	99.8
Redundancy	8.2	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		296	RESERVOIR SOLUTION: 100 MM BICINE PH 8, 150 MM NACL, 2.4 M (NH4)2SO4. VAPOR DIFFUSION AT 296 K WITH DROP RATIO OF 1.2 TO 0.8 UL PROTEIN TO RESERVOIR. PROTEIN CONCENTRATION: 4.9 MG/ML. POST-CRYSTALLIZATION SOAKING SOLUTION: 100 MM GLYCINE PH 10, 2.6 M (NH4)2SO4 PH 10, 25% SUCROSE.