5G33
Structure of Rad14 in complex with acetylnaphtyl-guanine containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-18 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 41 |
Unit cell lengths | 53.369, 53.369, 131.252 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.370 - 2.400 |
R-factor | 0.25341 |
Rwork | 0.251 |
R-free | 0.29832 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a3d |
RMSD bond length | 0.011 |
RMSD bond angle | 1.603 |
Data reduction software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.400 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.190 | 1.390 |
Number of reflections | 14071 | |
<I/σ(I)> | 5.8 | 1.1 |
Completeness [%] | 98.0 | 98 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |