5G2R
Crystal structure of the Mo-insertase domain Cnx1E from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-04 |
| Detector | RIGAKU CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.880, 123.410, 130.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.940 - 2.450 |
| R-factor | 0.2344 |
| Rwork | 0.233 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fc5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.462 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.060 | 0.890 |
| Number of reflections | 19069 | |
| <I/σ(I)> | 16.31 | 2 |
| Completeness [%] | 95.5 | 92.1 |
| Redundancy | 4.71 | 4.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.05 M LITHIUM SULFATE; 15 %(V/V) PEG 600; 10 % (V/V) GLYCEROL; 0.1 M HEPES, PH 7.5 |
