5G2F
Type IV-like competence pilin TTHA1222 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2012-07-14 |
| Detector | PILATUS |
| Spacegroup name | I 41 |
| Unit cell lengths | 84.380, 84.380, 97.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.720 - 1.850 |
| R-factor | 0.18421 |
| Rwork | 0.182 |
| R-free | 0.20781 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.492 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.860 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.030 | 0.680 |
| Number of reflections | 28582 | |
| <I/σ(I)> | 34.2 | 3.1 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 6.9 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA/K TARTRATE 0.1M SODIUM CITRATE PH 5.6 2.0 M AMMONIUM SULFATE |
