5G2D
The crystal structure of light-driven chloride pump ClR (T102N) mutant at pH 4.5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C |
Synchrotron site | PAL/PLS |
Beamline | 5C |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.252, 49.447, 78.402 |
Unit cell angles | 90.00, 131.89, 90.00 |
Refinement procedure
Resolution | 58.360 - 1.800 |
R-factor | 0.17674 |
Rwork | 0.175 |
R-free | 0.20524 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x3b |
RMSD bond length | 0.023 |
RMSD bond angle | 1.902 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.780 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.720 |
Number of reflections | 27489 | |
<I/σ(I)> | 22.67 | 2 |
Completeness [%] | 96.1 | 81.9 |
Redundancy | 4.6 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | pH 4.5 |