5G2D
The crystal structure of light-driven chloride pump ClR (T102N) mutant at pH 4.5.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C |
| Synchrotron site | PAL/PLS |
| Beamline | 5C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.252, 49.447, 78.402 |
| Unit cell angles | 90.00, 131.89, 90.00 |
Refinement procedure
| Resolution | 58.360 - 1.800 |
| R-factor | 0.17674 |
| Rwork | 0.175 |
| R-free | 0.20524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3b |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.780 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.720 |
| Number of reflections | 27489 | |
| <I/σ(I)> | 22.67 | 2 |
| Completeness [%] | 96.1 | 81.9 |
| Redundancy | 4.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
