5G2C
The crystal structure of light-driven chloride pump ClR (T102D) mutant at pH 4.5.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.441, 50.033, 77.786 |
Unit cell angles | 90.00, 131.47, 90.00 |
Refinement procedure
Resolution | 58.280 - 2.310 |
R-factor | 0.19692 |
Rwork | 0.195 |
R-free | 0.23878 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x3b |
RMSD bond length | 0.019 |
RMSD bond angle | 1.910 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.420 |
Number of reflections | 13298 | |
<I/σ(I)> | 14.42 | 1.83 |
Completeness [%] | 94.8 | 73.5 |
Redundancy | 4.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | pH 4.5 |