5G2A
The crystal structure of light-driven chloride pump ClR at pH 6.0 with Bromide ion.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-11-28 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.774, 48.850, 69.363 |
| Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
| Resolution | 65.130 - 2.170 |
| R-factor | 0.15583 |
| Rwork | 0.153 |
| R-free | 0.20714 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3b |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 17312 | |
| <I/σ(I)> | 35.45 | 5.26 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 5.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
