5G25

Type IV-like pilin TTHA1218 from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	77
Collection date	2012-10-01
Spacegroup name	F 41 3 2
Unit cell lengths	156.590, 156.590, 156.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.410 - 2.300
R-factor	0.20962
Rwork	0.208
R-free	0.23690
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0124)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.360	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.050	0.750
Number of reflections	7765
<I/σ(I)>	30.1	3.6
Completeness [%]	99.9	99.8
Redundancy	11.6	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M HEPES PH 7.0 1M SUCCINIC ACID 1% PEG 2000 MME
1				0.1 M HEPES PH 7.0 1M SUCCINIC ACID 1% PEG 2000 MME