5G24
Type IV-like pilin TTHA1219 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 77 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.830, 32.170, 108.120 |
| Unit cell angles | 90.00, 115.51, 90.00 |
Refinement procedure
| Resolution | 97.580 - 2.300 |
| R-factor | 0.19266 |
| Rwork | 0.190 |
| R-free | 0.24034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g23 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.877 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.770 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.820 |
| Number of reflections | 16586 | |
| <I/σ(I)> | 14.3 | 2.6 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.01M ZINC CHLORIDE, 0.1M SODIUM ACETATE PH 5 AND 20% (W/V) PEG 6000 |
