5G23
Type IV-like pilin TTHA1219 from Thermus thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 77 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.400, 66.950, 97.470 |
| Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
| Resolution | 96.230 - 1.850 |
| R-factor | 0.18173 |
| Rwork | 0.180 |
| R-free | 0.20878 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.940 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.790 |
| Number of reflections | 70620 | |
| <I/σ(I)> | 12.6 | 2.2 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.01M ZINC CHLORIDE, 0.1M SODIUM ACETATE PH 5 AND 20% (W/V) PEG 6000 |
