5G1V
Linalool Dehydratase Isomerase: Selenomethionine Derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Collection date | 2015-09-19 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.506, 106.716, 222.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.410 - 2.680 |
| R-factor | 0.16967 |
| Rwork | 0.168 |
| R-free | 0.20345 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.872 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.510 | 2.740 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.240 | 0.730 |
| Number of reflections | 68022 | |
| <I/σ(I)> | 10.8 | 3.9 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 13 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.1 M SODIUM TARTATE PH 7.0; 1% (V/V) GLYCEROL; PROTEIN AT 10 MG PER ML |
