5G1Q

Compressed conformation of Francisella tularensis ClpP at 2.84 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Detector	RIGAKU CCD
Spacegroup name	P 21 21 2
Unit cell lengths	113.461, 125.940, 96.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.950 - 2.840
R-factor	0.25239
Rwork	0.249
R-free	0.32604
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p2l
RMSD bond length	0.008
RMSD bond angle	1.243
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.950	2.970
High resolution limit [Å]	2.840	2.840
Rmerge	0.100	0.340
Number of reflections	32741
<I/σ(I)>	5.9	2.2
Completeness [%]	97.8	98.8
Redundancy	2.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	RATIO 1:1, 293K, SITTING DROP. RESERVOIR CONDITION: 0.2 M NACL, 30% (V/V) MPD AND 0.1 M SODIUM ACETATE PH 4.6