5G1E

The complex structure of syntenin-1 PDZ domain with c-terminal extension

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	77
Detector technology	CCD
Spacegroup name	P 21 21 21
Unit cell lengths	52.740, 63.318, 103.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.030 - 1.920
R-factor	0.23399
Rwork	0.232
R-free	0.27983
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.708
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.050	0.350
Number of reflections	27216
<I/σ(I)>	41.2	4.3
Completeness [%]	97.9	99.3
Redundancy	10.7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M BIS-TRIS (PH 6.5), 25% (W/V) POLYETHYLENE GLYCOL 3350