5G13
Pseudomonas aeruginosa HDAH (H143A) unliganded.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-18 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.495, 81.495, 205.265 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.740 - 1.990 |
R-factor | 0.17484 |
Rwork | 0.173 |
R-free | 0.21723 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g0x |
RMSD bond length | 0.009 |
RMSD bond angle | 1.323 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.740 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.180 | 0.340 |
Number of reflections | 48272 | |
<I/σ(I)> | 11.2 | 4.8 |
Completeness [%] | 99.5 | 95.9 |
Redundancy | 15.5 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |