5G10
Pseudomonas aeruginosa HDAH bound to 9,9,9 trifluoro-8,8-dihydroy-N-phenylnonanamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.740, 81.740, 205.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.940 - 1.710 |
| R-factor | 0.15967 |
| Rwork | 0.158 |
| R-free | 0.19366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g0x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.940 | 1.750 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.150 | 1.180 |
| Number of reflections | 75585 | |
| <I/σ(I)> | 8.2 | 2.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 7.7 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
