5G0Z

Structure of native granulovirus polyhedrin determined using an X-ray free-electron laser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	293
Detector technology	PIXEL
Collection date	2013-02-10
Detector	CORNELL-SLAC PIXEL ARRAY DETECTOR
Wavelength(s)	1.5600
Spacegroup name	I 2 3
Unit cell lengths	103.400, 103.400, 103.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.557 - 2.001
R-factor	0.1517
Rwork	0.148
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jvb
RMSD bond length	0.006
RMSD bond angle	0.739
Data reduction software	CrystFEL (V. 0.5.2)
Data scaling software	CrystFEL (V. 0.5.2)
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	12600
<I/σ(I)>	9.59	0.92
Completeness [%]	100.0	100
Redundancy	6008	1258
CC(1/2)	0.997	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				IN VIVO