5G0Y
Pseudomonas aeruginosa HDAH unliganded.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.530, 81.530, 203.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.670 - 2.290 |
R-factor | 0.18898 |
Rwork | 0.187 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zz0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.359 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.670 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.230 | 0.970 |
Number of reflections | 31717 | |
<I/σ(I)> | 5.3 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.5 |