5FYO

Calcium-dependent phosphoinositol-specific phospholipase C from a Gram-negative bacterium, Pseudomonas sp, apo form, crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-02
Detector	DECTRIS PILATUS3 6M
Spacegroup name	P 43 2 2
Unit cell lengths	96.080, 96.080, 113.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.810 - 1.500
R-factor	0.12691
Rwork	0.125
R-free	0.16673
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h4w
RMSD bond length	0.015
RMSD bond angle	1.575
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.810
High resolution limit [Å]	1.500
Rmerge	0.050
Number of reflections	85192
<I/σ(I)>	27.5
Completeness [%]	99.9
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10		PEG3350 24-27%, CAPS/BICINE MIX PH 9.0-10 GRID, BEST CRYSTALS CLOSER TO PH 10, SEEDING FROM PACT H4 (0.2M KSCN, 20% PEG3350, BIS TRIS PROPANE PH 8.5), ORYX ROBOT, 24 WELL PLATE, HANGING DROPS