5FYA

Cubic crystal of the native PlpD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-08
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 3
Unit cell lengths	145.906, 145.906, 145.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.635 - 2.141
R-factor	0.2013
Rwork	0.199
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fqu
RMSD bond length	0.004
RMSD bond angle	0.787
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	2.270
High resolution limit [Å]	2.140	2.140
Number of reflections	54378
<I/σ(I)>	61.6	3
Completeness [%]	95.3	85.4
Redundancy	5.9	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				1.4 M MAGNESIUM SULFATE, 0.1M BIS-TRIS PROPANE PH 7.0