5FXW
Crystal structure of JmjC domain of human histone demethylase UTY in complex with fumarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.630, 109.440, 118.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.720 - 2.090 |
R-factor | 0.1947 |
Rwork | 0.192 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zli |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | DIMPLE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.720 | 2.140 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.130 | 1.450 |
Number of reflections | 69482 | |
<I/σ(I)> | 11 | 1.2 |
Completeness [%] | 98.9 | 99.9 |
Redundancy | 6.2 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.8 | 0.1M HEPES PH 7.8 -- 0.25M TRIMETHYLAMINE N-OXIDE -- 15% PEG3350 |