5FXW
Crystal structure of JmjC domain of human histone demethylase UTY in complex with fumarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.630, 109.440, 118.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.720 - 2.090 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.720 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.130 | 1.450 |
| Number of reflections | 69482 | |
| <I/σ(I)> | 11 | 1.2 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 0.1M HEPES PH 7.8 -- 0.25M TRIMETHYLAMINE N-OXIDE -- 15% PEG3350 |
