5FXF
Crystal structure of eugenol oxidase in complex with benzoate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.290, 96.870, 179.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.830 - 1.900 |
| R-factor | 0.16722 |
| Rwork | 0.165 |
| R-free | 0.21199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vao |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.921 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.830 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.150 | 0.980 |
| Number of reflections | 79217 | |
| <I/σ(I)> | 8.6 | 2.7 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24% PEG6000, 0.1 M TRIS/HCL PH 8.0 |
